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w v non fat dried skimmed milk powder  (Bio-Rad)


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    Bio-Rad w v non fat dried skimmed milk powder
    W V Non Fat Dried Skimmed Milk Powder, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 15901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/w v non fat dried skimmed milk powder/product/Bio-Rad
    Average 98 stars, based on 15901 article reviews
    w v non fat dried skimmed milk powder - by Bioz Stars, 2026-06
    98/100 stars

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    Representative ITC thermograms (top panel) and corresponding integrated binding isotherms (bottom panel) for (A) WT FGF1 and (B) FLEX titrated with polymeric heparin from three independent experiments. Experiments were performed at 25 °C in Tris pH 7.4 with 100 mM NaCl. Data were fitted using a single-site binding model.

    Journal: bioRxiv

    Article Title: FLEX: A heparin-binding fusion partner engineered from fibroblast growth factor 1 to enhance protein expression, solubility and purity

    doi: 10.64898/2026.03.17.712271

    Figure Lengend Snippet: Representative ITC thermograms (top panel) and corresponding integrated binding isotherms (bottom panel) for (A) WT FGF1 and (B) FLEX titrated with polymeric heparin from three independent experiments. Experiments were performed at 25 °C in Tris pH 7.4 with 100 mM NaCl. Data were fitted using a single-site binding model.

    Article Snippet: Membranes were blocked in Tris-buffered saline with 0.1% (v/v) Tween 20 (TBS-T) in 5% (w/v) non-fat dried milk (pH 7.4) followed by incubation with primary anti-6His tag antibody (Bio-Rad or Cell Signalling Technologies) and secondary horse radish peroxidase-conjugated anti-mouse (ThermoFisher) antibody.

    Techniques: Binding Assay